Effects of formic acid decalcification on Luxol Fast Blue/Cresyl violet for myelin

From:Gayle Callis

Dear Histonetters, 

Thanks for answers on decalcification of mouse vertebral column to stain
spinal cord (rather than push cord out with PBS) - decalcification was a
consideration here also. It has been ages since I did Luxol fast
blue/cresyl violet staining so here is the next question

Will formic acid decalcification affect the staining quality or compromise
staining of Luxol Fast blue/cresyl violet for myelin, other CNS tissues? If
so, I go for cord removal to get rid of bone. 


Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
S. 19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

email: gcallis@montana.edu

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