Dear Histonetters, 

Thanks for answers on decalcification of mouse vertebral column to stain
spinal cord (rather than push cord out with PBS) - decalcification was a
consideration here also. It has been ages since I did Luxol fast
blue/cresyl violet staining so here is the next question

Will formic acid decalcification affect the staining quality or compromise
staining of Luxol Fast blue/cresyl violet for myelin, other CNS tissues? If
so, I go for cord removal to get rid of bone. 

  


Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
S. 19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

email: gcallis@montana.edu