Dunn-Thompson method

From:Deborah Faichney

I have been asked to stain for possible Haemoglobin in sections. Never done
this before!!  I have found the Dunn-Thompson method (1945) in Carleton's
histological technique.  

It states ' stain in a ripened aqueous solution of alum haematoxylin (0.25%
in 5% alum for 15 minutes).  Any unacidified haematoxylin solution may be

Is Mayer's an acceptable Alum haematoxylin for use in this method? This is
the one we routinely use for our H&E.

How do I prepare the working Haematoxylin stain? 

Are there other methods that you favour?
Many thanks 

Debbie Faichney

Institute of Aquaculture
University of Stirling
Stirling, Fk9 4LA


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