I have been asked to stain for possible Haemoglobin in sections. Never done
this before!!  I have found the Dunn-Thompson method (1945) in Carleton's
histological technique.  


It states ' stain in a ripened aqueous solution of alum haematoxylin (0.25%
in 5% alum for 15 minutes).  Any unacidified haematoxylin solution may be
used.'

Is Mayer's an acceptable Alum haematoxylin for use in this method? This is
the one we routinely use for our H&E.

How do I prepare the working Haematoxylin stain? 

Are there other methods that you favour?
 
Many thanks 

Debbie Faichney

Histopathology
Institute of Aquaculture
University of Stirling
Stirling, Fk9 4LA
Scotland

daf3@stir.ac.uk

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