I don't know if this subject has been addressed before, but, here goes
anyway!
 
Our lab is using disposable blades (low profile).  We currently have one
Leica 2135 microtome and four Leitz 1512 .  The Leica is used with
disposable blades and two of the Leitz are also used with the disposable
blades using 2 differant blade holders.  We are having a horrible problem
with uneven cut sections which in turn  causes the tissue to stain unevenly.
After pulling most of my hair out, I I really do not believe it is a
processing problem or a stain problem.  And, it is'nt all of the tissues
being affected this way.  Some are beautiful and some are terrible.  We have
tightened screws and bolts and we also just had all of our microtomes
serviced.  
 
Any suggestions?  I would appreciate your help.
 
Anita Westbrook
 


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