Alternative to Vectashield
Hello histonetters.
We're doing a lot of fluorescent staining on 40 micron, perfusion
fixed, vibrating microtome cut, mouse brain sections. We're using FITC,
Rhodamine Red-X, and Cy5 conjugated secondary antibodies. The IHC is
going pretty well, but we are going through a lot of Vectashield
mounting medium from Vector Labs. It works well, but it's getting a bit
pricey. Does anyone know of a cheap, alternative mounting medium for
fluorescence? Any help would be appreciated. Thanks.
Matt
Matt McElwee
Research Specialist
Department of Surgery
K4/617 Clinical Science Center
600 Highland Avenue
Madison, WI 53792
(608) 263-7648
mcelwee@surgery.wisc.edu
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