toluidine again

From:Jaclynn Lett

Regarding my Toluidine Blue stain solution, which should be staining animal
tissue varying shades of blue-purple (which I want), but is now staining
bright blue instead (which I don't want):

1% Toluidine Blue O
1% Sodium Tetraborate Dodecahydrate
30% Ethanol
in DDI

(1g. Tol.Blue, 1g Borax, 30mL Ethanol in 100mL DDI)

I've received several suggestions to change the pH--some to acidic, some to
basic.  Which direction should it be changed and what chemical do I use to
change it?

Thank you again,

Jaclynn M. Lett, Research Technician
Electron Microscopy Core Facility
Fay and Carl Simons Center for Biology of Hearing and Deafness
Central Institute for the Deaf
4560 Clayton Ave.
St. Louis, MO 63110

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