From: | Joan Baizer |
Hello. I need some advice about cell staining on very old human brain tissue. The brain was embedded in celloidin and the blocks and some cut sections stored in 70% alcohol (about 40 years). I have not gotten good staining with cresyl, Giemsa or netural red, using very standard protocols. Does anyone know of a way to impove staining of old overfixed brain? thanks Joan Baizer University at Buffalo