The answer to the question is yes, background will be a huge problem. 

Try a mouse on mouse kit, Zymed, Innogenex, Scytek, Vector all have special
kits containing special blockers. I hope I haven't missed a vendor, if so,
speak up if you have the kit.

Another alternative is DAKO ARK kit, based on biotinylation of the primary
antibody and their special blocking, etc.

Some people have tried more than one kit for success, they do vary - but
you may be lucky with first one you try. 

  


Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367 (voice mail)
406 994-4303 (FAX)

email: UVSGC@montana.edu