Histonetters,
I'm staining some sections of cells grown on membranes to see alpha and 
beta cells using Gomori's Method for Pancreatic Islet Cells, green AFIP 
manual. The investigator has requested this particular stain and I have not 
done this one before. My question is about mordanting in Bouins for 16 hrs. 
The green AFIP manual doesn't specify temperature so I am assuming room 
temp. Can this be shortened by heating the Bouins to 56 degrees like you do 
for the Masson Trichrome?
Thanks!
Andi Grantham
.....................................................................
: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
: Sr. Research Specialist       University of Arizona               :
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