Anyone out there doing immunostaining on WT1 protein for Wilms' Tumor
I am trying out this new antibody (Dako cat # M3561)and really donot
want to give up yet! I am using a mesothelioma as a control on formalin
fixed paraffin embedded tissue (FFPET).
the protocol suggests pretreating the slides with pepsin in0.2N HCl and
the immunolocalisation should be nuclear. I have tried this digestion as
well using mw antigen retrieval using several antigen retrieval buffers;
I keep getting peri-nuclear and cytoplasmic staining.
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