Toluidine Blue.
| From: | "Dr. Ian Montgomery" |
Jaclynn,
I've been
using this stain for more than 30 years and it works beautifully.
Sodium borate. = 0.8g
Distilled water. = 100ml
Dissolve, then add in order
Toluidine blue. = 0.8g
Pyronin
Y.
= 0.2g
Dissolve, then filter into a bottle.
Stain sections at room temperature until the desired depth of staining is
obtained (few minutes or more). Wash in running tap water, air dry and
mount in immersion oil or a synthetic mountant. The stain gives a very
pleasing result of varying shades of blue.
Ito,S. & Winchester,R.J. 1963. J. Cell Biol. 16. 541-577
The fine structure of the gastric mucosa in the bat.
Ian.
Dr. Ian Montgomery,
Histotechnology,
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
Glasgow,
G12 8QQ.
Tel: 0141 339 8855
Office: 4652
Lab: 6644.
e-mail: ian.montgomery@bio.gla.ac.uk
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