Re: stain for tyrosine dehydroxylase

From:Geoff McAuliffe

Hi Nancy:

    Do you mean tyrosine hydroxylase, the enzyme that is the rate-limiting step
in catecholamine synthesis? Basal ganglia are loaded with it. If so, formalin
fixed frozen sections give very good results. Paraffin sections are a maybe,
cell bodies with lots of TH yes, fine fibers probably not.


Nancy Maronto wrote:

> Hi,
> I am looking for a stain to demonstrate tyrosin dehydroxylase.  This will be
> done on the basal ganglia on animal tissue.  Would the tissue need to be
> fresh frozen, fixed frozen, or formalin fixed?  Any staining tips with the
> staining protocol would be appreciated.  Thanks for your help.
> Nancy Maronto
> MPI Research
> Mattawan, MI
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

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