Re: stain for tyrosine dehydroxylase
Hi Nancy:
Do you mean tyrosine hydroxylase, the enzyme that is the rate-limiting step
in catecholamine synthesis? Basal ganglia are loaded with it. If so, formalin
fixed frozen sections give very good results. Paraffin sections are a maybe,
cell bodies with lots of TH yes, fine fibers probably not.
Geoff
Nancy Maronto wrote:
> Hi,
> I am looking for a stain to demonstrate tyrosin dehydroxylase. This will be
> done on the basal ganglia on animal tissue. Would the tissue need to be
> fresh frozen, fixed frozen, or formalin fixed? Any staining tips with the
> staining protocol would be appreciated. Thanks for your help.
>
> Nancy Maronto
> MPI Research
> Mattawan, MI
>
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--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
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