I have just placed an order this morning. Will receive the reagents tomorrow to
test this out.  You are absolutely right this could be a huge move in mouse on
mouse technology also on double-labeling with two monoclonal antibodies.
Barb:)

Robert Geske wrote:

> i haven't tried this yet, but i think it could be HUGE !!!! Molecular Probes
> has excellent reagents and if it works as well as other products of theirs i
> have used it could be the answer to many many a prayer to the histology
> gods.
>
> Go to www.probes.com and look for a link to "Zenon Technology".
>
> if anyone has tried this, please let us know the results.
>
> rob
>
> -----Original Message-----
> From: Gayle Callis [mailto:uvsgc@montana.edu]
> Sent: Tuesday, May 28, 2002 11:13 AM
> To: histonet@pathology.swmed.edu
> Subject: mouse monoclonal on mouse tissue
>
> The answer to the question is yes, background will be a huge problem.
>
> Try a mouse on mouse kit, Zymed, Innogenex, Scytek, Vector all have special
> kits containing special blockers. I hope I haven't missed a vendor, if so,
> speak up if you have the kit.
>
> Another alternative is DAKO ARK kit, based on biotinylation of the primary
> antibody and their special blocking, etc.
>
> Some people have tried more than one kit for success, they do vary - but
> you may be lucky with first one you try.
>
>
>
> Gayle Callis
> MT,HT,HTL(ASCP)
> Research Histopathology Supervisor
> Veterinary Molecular Biology - Marsh Lab
> Montana State University - Bozeman
> 19th and Lincoln St
> Bozeman MT 59717-3610
>
> 406 994-6367 (voice mail)
> 406 994-4303 (FAX)
>
> email: UVSGC@montana.edu
>
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