Re: mouse monoclonal on mouse tissue
Good point, we have also used biotinylated primary antibodies, monoclonals,
but not with Envision+ with standard protocols and some special normal
serum blocking and careful normal serum blocking.
Mouse on mouse kits were just a suggestion.
At 10:15 AM 5/28/02 -0700, you wrote:
>i beg to differ gayle. i've done several mouse-on-mouse (most recent
>notable is Ki-67 (clone MM1)) that have no or very little background using
>our standard protocol (using Dako Envision+/DAB) with no additional
>blocking. i find it less time consuming if i try the aby first with no
>special protocol and only go to the "mouse-on-mouse" stuff if absolutely
>Carrie Kyle-Byrne, BHS, HT(ASCP)
>Assoc. Research Scientist II
>Molecular Target Research
>170 Harbor Way
>P.O. Box 511
>South San Francisco
>CA 94083-0511 USA
>Phone: (1 650) 837-8023
>Fax: (1 650) 837-7240
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>----- Original Message -----
>From: "Gayle Callis"
>Sent: Tuesday, May 28, 2002 9:12 AM
>Subject: mouse monoclonal on mouse tissue
>> The answer to the question is yes, background will be a huge problem.
>> Try a mouse on mouse kit, Zymed, Innogenex, Scytek, Vector all have
>> kits containing special blockers. I hope I haven't missed a vendor, if so,
>> speak up if you have the kit.
>> Another alternative is DAKO ARK kit, based on biotinylation of the primary
>> antibody and their special blocking, etc.
>> Some people have tried more than one kit for success, they do vary - but
>> you may be lucky with first one you try.
>> Gayle Callis
>> Research Histopathology Supervisor
>> Veterinary Molecular Biology - Marsh Lab
>> Montana State University - Bozeman
>> 19th and Lincoln St
>> Bozeman MT 59717-3610
>> 406 994-6367 (voice mail)
>> 406 994-4303 (FAX)
>> email: UVSGC@montana.edu
Research Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
19th and Lincoln St
Bozeman MT 59717-3610
406 994-6367 (voice mail)
406 994-4303 (FAX)
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