Thanks Mary!
I'll let you know how things work.
Anne Undersander HT(ASCP)
Vet. Med./Animal Science
University of Minnesota
----- Original Message -----
From: "Georger, Mary" 
To: "'Anne Undersander'" 
Sent: Thursday, May 30, 2002 1:21 PM
Subject: RE: iNOS,eNOS,nNOS


> Anne,
> We buy our iNOS from Chemicon, #AB5382  and our eNOS from Transduction
> Laboratories, #N30020. I haven't a reliable nNOS so I'm afraid I can't
help
> you out there.
> Best of luck!
> I will fax out the protocols within the hour.
>
> Mary Georger
> Center for Cardiovascular Research
> University of Rochester Medical Center
> KMRB Room 2-9816
> 601 Elmwood Avenue.
> PO Box 679
> Rochester, New York  14642
> 585-273-1548
>
> > ----------
> > From: Anne Undersander
> > Sent: Thursday, May 30, 2002 12:07 PM
> > To: Georger, Mary
> > Subject: Re: iNOS,eNOS,nNOS
> >
> > Thanks Mary.
> >
> > The fax number here is 612-625-0204 just address it to me.  I really
> > appreciate your help.  One other thing, where do you get your antibodies
> > from?
> >
> > Thanks again.
> > Anne Undersander
> > Vet Med/Animal Science
> > University of Minnesota
> > ----- Original Message -----
> > From: "Georger, Mary" 
> > To: "'Anne Undersander'" 
> > Sent: Thursday, May 30, 2002 9:10 AM
> > Subject: RE: iNOS,eNOS,nNOS
> >
> >
> > > Anne,
> > > Do you have a fax number where I can send you my protocols?
> > > We fix in 10% NBF for 24 hours, no longer, but we are doing rodent
> > tissues.
> > > You may have an issue with size on horse tissue. My protocol sheets
have
> > all
> > > the antigen retrieval info on them.
> > > Let me know.
> > > Mary Georger
> > > Center for Cardiovascular Research
> > > University of Rochester Medical Center
> > > KMRB Room 2-9816
> > > 601 Elmwood Avenue.
> > > PO Box 679
> > > Rochester, New York  14642
> > > 585-273-1548
> > >
> > > > ----------
> > > > From: Anne Undersander
> > > > Sent: Thursday, May 30, 2002 9:59 AM
> > > > To: HistoNet Server
> > > > Subject: iNOS,eNOS,nNOS
> > > >
> > > > Histonet (Mary Georger),
> > > >
> > > > I was asked to perform IHC against each isoform(iNOS,eNOS,nNOS) on
> > FFPE
> > > > horse uterine biopsies.  I've searched the archives and found some
> > > > information, although none on horse.  I need any/all information I
can
> > get
> > > > regarding protocols, fixation and especially positive controls.
> > Please
> > > > help.  I would appreciate any info.
> > > > Thanks.
> > > > Anne Undersander
> > > > Vet. Med/Animal Science
> > > > University of Minnesota
> > > > 612 625 7758
> > > >
> > >
> >
> >
>