We are using the following protocol for CD25 on formalin fixed, paraffin
embedded tissue

Antibody: CD25
Clone: 4C9
Manufacturer: Novocastra (distributed by Vector)
Pretreatment: Steam 40' in EDTA, pH8
Dilution: 1:1000   
Detection: Envision+

Patti Loykasek
Phenopath Laboratories
Seattle, WA

> From: "Dawson, Glen" 
> Date: Wed, 22 May 2002 11:43:17 -0500
> To: SMBJ123@aol.com, histonet@pathology.swmed.edu
> Subject: RE: cd25 on paraffin sections
> 
> I use Novocastra CD25 (#NCL-CD25) distributed by Vector.  LSAB+ detection
> following 40 min. target retrieval in pH6.0 DAKO target retrieval @ 100
> degree C.  It works well.
> 
> Good Luck,
> 
> Glen Dawson 
> 
> 
> 
> -----Original Message-----
> From: SMBJ123@aol.com [mailto:SMBJ123@aol.com]
> Sent: Wednesday, May 22, 2002 10:58 AM
> To: histonet@pathology.swmed.edu
> Subject: cd25 on paraffin sections
> 
> 
> Is anyone doing CD25 on paraffin sections or is it only done on frozen
> sections or flow?
> Sally Jordan
> Dermatopathology Associates
> Jackson, MS
>