I have actually prefer the mordant step overnight with Bouins at RT (for
unstable, huge decalcified bone sections) to avoid hot, nasty smelling
reagent.  It gave excellent, unchanged Masson Tri results, same as heated
method at 60 min, 56C, so I presume it would not make any difference on
long 16 hour RT or heated mordant step. I gave up using a (long ago
inexperienced use of)  microwave to do this step after blasting Bouins all
over the inside of MW, clean up was not pleasant plus Jerry Fredenburgh
later discussed MW mordanting as being too short for adequate staining
results in his staining workshop, a chemical consideration.



 


Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367 (voice mail)
406 994-4303 (FAX)

email: UVSGC@montana.edu