Re: Resin suggestions.

From:Geoff McAuliffe


    An Epon substitute or "American" Araldite (Araldite 502:DDSA at 27:23). With Epon substitutes, at least the ones I have used, you can skip the 100% EtOH by going from 95% to a 95%-Epon mix. Epon does mix with ethanols, just more slowly than do the usual "clearing" agents. I have heard of going from 70% EtOH to Epon! I am told that Spurr is too highly crosslinked for good immuno.

"Dr. Ian Montgomery" wrote:

What is the current resin of choice for IHC on 1 micron sections. I've not carried out the method for many years so would appreciate any suggestions. At the time I tried LR White but was not impressed, has the situation improved.

Dr. Ian Montgomery,
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
G12 8QQ.
Tel: 0141 339 8855
Office: 4652
Lab: 6644.

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

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