something I've wondered about and would appreciate your thoughts.
I would think that those amino groups want to attract anything that will
neutralize their charge and couldn't this happen long before that tissue
section ends up on the slide?
Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical
University of South Carolina
165 Ashley Avenue Suite 309
Charleston,
SC 29425
Ph: 843-792-6353
fax: 843-792-8974
>>> "J. A.
Kiernan" <jkiernan@uwo.ca> 05/29/02 03:06PM >>>
rueggp
wrote:
>
> are APES coated slides available for sale already
prepared or do you
> have to coat them yourself in house.
APES
(aminopropyltriethoxysilane) does not coat the slide;
it reacts chemically
with the silicate of the glass.
The treatment with APES chemically binds
amino groups to the
surface of the glass, which consequently becomes
positively
charged when wetted with water.
Positively charged slides
are sold ready-made under
various trade names. I don't know if they are made
with
APES or with some other ethoxysilane that has a basic
side-chain.
--
-------------------------
John A.
Kiernan
Department of Anatomy and Cell Biology
The University of Western
Ontario
London, Canada N6A 5C1
kiernan@uwo.ca
http://publish.uwo.ca/~jkiernan/