> are APES coated slides available for sale already prepared or do you
> have to coat them yourself in house.
APES (aminopropyltriethoxysilane) does not coat the slide;
it reacts chemically with the silicate of the glass.
The treatment with APES chemically binds amino groups to the
surface of the glass, which consequently becomes positively
charged when wetted with water.
Positively charged slides are sold ready-made under
various trade names. I don't know if they are made with
APES or with some other ethoxysilane that has a basic
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London, Canada N6A 5C1
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