Hi Netters.
Using monoclonals on mouse tissue also depends on the mouse strain that
you're using.  We have great success using monoclonal antibodies on
SCID/beige mouse tissue because they're immune deficient.  Nude mice are
immune compromised (they only have B cells) and so we use the DAKO ARK kit
and it works great - very clean.
Jennifer




-----Original Message-----
From: Gayle Callis [mailto:uvsgc@montana.edu]
Sent: Tuesday, May 28, 2002 9:13 AM
To: histonet@pathology.swmed.edu
Subject: mouse monoclonal on mouse tissue


The answer to the question is yes, background will be a huge problem. 

Try a mouse on mouse kit, Zymed, Innogenex, Scytek, Vector all have special
kits containing special blockers. I hope I haven't missed a vendor, if so,
speak up if you have the kit.

Another alternative is DAKO ARK kit, based on biotinylation of the primary
antibody and their special blocking, etc.

Some people have tried more than one kit for success, they do vary - but
you may be lucky with first one you try. 

  


Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367 (voice mail)
406 994-4303 (FAX)

email: UVSGC@montana.edu


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