i haven't tried this yet, but i think it could be HUGE !!!! Molecular Probes
has excellent reagents and if it works as well as other products of theirs i
have used it could be the answer to many many a prayer to the histology
gods.  

Go to www.probes.com and look for a link to "Zenon Technology".  


if anyone has tried this, please let us know the results.


rob

-----Original Message-----
From: Gayle Callis [mailto:uvsgc@montana.edu]
Sent: Tuesday, May 28, 2002 11:13 AM
To: histonet@pathology.swmed.edu
Subject: mouse monoclonal on mouse tissue


The answer to the question is yes, background will be a huge problem. 

Try a mouse on mouse kit, Zymed, Innogenex, Scytek, Vector all have special
kits containing special blockers. I hope I haven't missed a vendor, if so,
speak up if you have the kit.

Another alternative is DAKO ARK kit, based on biotinylation of the primary
antibody and their special blocking, etc.

Some people have tried more than one kit for success, they do vary - but
you may be lucky with first one you try. 

  


Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367 (voice mail)
406 994-4303 (FAX)

email: UVSGC@montana.edu



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