RE: WEIRD FIXATIVE - Analyzed Weirdly
|From:||"Monson, Frederick C." |
And what is the probability that our "research tech" substituted good old
cupric sulfate? With respect to the "turquoise" haze, I'd bet on the rinse
in buffer or water to see it dissipate. On the other hand, Bryan is correct
about the mordanting. Gray without comment supports the notion that if it
could be found anywhere, someone, from HISTORY!!!!! - tried to fix or color
or mount with it.
Perhaps after Andrea gets sections she will prepare the best hematoxylin
stains she has ever had.
What I liked most was the reference to the neighborhood of Zn. Did s/he
believe that Zn was more related to Cu or was there no Cd. I would probably
have gone for the Ga, since I generally lean to the right anyway. It is
clear that the research tech has a tendency to lean to the left. Perhaps
someone has another, non-political explanation for this phenomenon.
This is great fun! Friday is upon us!
Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Schmucker II Science Center
West Chester University
South Church Street and Rosedale
West Chester, Pennsylvania, USA, 19383
CASI URL: http://darwin.wcupa.edu/casi/
WCUPA URL: http://www.wcupa.edu/
Visitors URL: http://www.wcupa.edu/_visitors/
> From: Bryan Llewellyn
> Sent: Thursday, May 30, 2002 2:04 PM
> To: Histonet
> Subject: Re: WEIRD FIXATIVE
> Gray lists about 26 copper containing fixatives. Two of these are
> copper-formalin mixtures.
> Gelderd's (1909. Ref. Cellule, v25, p12) solution is:
> Sea water 250 mL
> Cupric nitrate 5 g.
> Sat. formalin 12.5 mL
> Stappers' (1909. Ref. Cellule, v25, p356) solution is:
> Water 220 mL
> Cupric nitrate 5 g.
> Sat. formalin 30 mL
> Gray comments:
> "Cupric salts are never used alone for fixation. They are good fixatives
> general, owing to their power of mordanting tissues, particularly those
> which it is desired subsequently to stain in hematoxylin."
> Gray, P..
> The microtomist's formulary and Guide, p219.
> Krieger, NY.
> ISBN: 0-88275-247-2
> Bryan Llewellyn
> ----- Original Message -----
> From: "Andrea Grantham"
> Sent: Thursday, May 30, 2002 8:17 AM
> Subject: WEIRD FIXATIVE
> > One of the research techs here brought some tumor tissue to the lab
> > in Copper Formalin.
> > In one of his replys to my quizzing him about the fixative he said that
> > wanted to make up zinc formalin but didn't have any zinc and "copper is
> > right next to zinc" (on the chart, I presume) so he just thought he'd
> > it a try.
> > What might we expect in terms of staining?
> > When we bisected the tumors to process it was interesting to see that
> > was a turquoise color in the center of the tissue. None of the other
> > fixed in other fixatives (10% NBF, Carnoys) had this color.
> > Andi Grantham
> > .....................................................................
> > : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
> > : Sr. Research Specialist University of Arizona :
> > : (office: AHSC 4212) P.O. Box 245044 :
> > : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA :
> > : (FAX: 520-626-2097) (email: email@example.com) :
> > :...................................................................:
> > http://www.cba.arizona.edu/histology-lab.html
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