Stephen,
What type of NOS are you evaluating? I have done extensive work with both
iNOS and eNOS with reliable results. However in my hands nNOS was a bit more
capricious. Let me know what type you are interested in  and I will gladly
share my protocols.

Mary Georger
Center for Cardiovascular Research
University of Rochester Medical Center
KMRB Room 2-9816
601 Elmwood Avenue.
PO Box 679
Rochester, New York  14642
585-273-1548

> ----------
> From: 	Stephen Asquith
> Sent: 	Tuesday, May 28, 2002 10:09 PM
> To: 	'HistoNet Server'
> Subject: 	Rabbit anti-Nos Antibodies
> 
> 	
> 	Dear all, we are currently using a non-commercial NOS antibody that
> has proved very reliable over the years but now we are coming to the end
> of
> it and the source is no longer available. Hence we are considering
> purchasing a commercial rabbit NOS antibody. Questions are, has anyone
> used
> either the Chemicon  AB1552 or the AB5380 antibodies on rat what do the
> results look like ( would you be happy to email me the images?) are there
> other commercial NOS antibodies worth considering. 
> Many thanks in advance
> Steve Asquith
> Anatomy & Cell Biology
> The University of Melbourne
> Telephone: + 613 8344 7424
> Facsimile: + 613 9347 5219
> 
> 
> 
> 
>