I have to disagree with Dorit's statement. Patsy's original advice is best
and will lead to fewer problems. Using a rabbit anti-mouse antibody in rabbit
tissue provides a GREATER possiblity of cross reactivity with other rabbit
antigens that could confuse the result. just as humans react immunologically to
antigens from transplanted human tissues, there is no reason to assume that one
rabbit's tissues would not contain antigens that another rabbit might have
antibodies in its serum that will react to those rabbit antigens.
Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical
University of South Carolina
165 Ashley Avenue Suite 309
Charleston,
SC 29425
Ph: 843-792-6353
fax: 843-792-8974
>>> Dorit
Zharhary <d_zharhary@sigma.co.il> 05/23/02 05:51AM >>>
On the
contrary. It will be best to use an anti-mouse made in rabbit
because
chances are low that this serum will contain any antibodies to
rabbit
proteins
-----Original Message-----
From: Robert
Geske [SMTP:RGeske@lexgen.com]
Sent: Thu 23 May 2002
1:50
To: 'rueggp'
Cc:
'histonet@pathology.swmed.edu'
Subject: RE: Mouse
anti-rabbit antibodies
"..........you should use an anti-mouse secondary
which is not made in
rabbit.".
Why ???
-----Original
Message-----
From: rueggp [
mailto:rueggp@earthlink.net]Sent:
Tuesday, May 21, 2002 2:02 PM
To: Julia Friedman
Cc:
histonet@pathology.swmed.edu
Subject: Re: Mouse anti-rabbit
antibodies
sometimes you just have to try your m<h antibody on the
rabbit tissue to
see
if it works. keep in mind that you should use
an anti-mouse secondary
which
is not made in rabbit. Do not use
a
universal secondary with anti-rabbit mixed with the anti-mouse. i
would
use
rabbit serum in my block as well. (10% serum from the animal
being stained)
patsy
Julia Friedman wrote:
> Does anyone
know of a source for mouse anti-rabbit CD4 & CD8? I will be
using them on
rabbit tissue. Do you think I could get away with mouse
anti-human CD4 and
CD8 on rabbit tissue?
Thanks.
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