RE: GFP fluorescence after formalin/paraffin

From:"Montague, Donna C"

The GFP fluorescence will survive paraffin processing IF the vector used
contains enhanced GFP (EGFP). Hint, do not counterstain your secetions with
eosin its fluorescence will interferr with that of the GFP. If you must use
a counterstain for orientation in light microscopy use heamatoxylin or other
non-fluorescent stain. Hope this helps. Donna Montague, M.S.
Research Associate
Physiology/Biophysics and Orthopaedic Surgery
University of Arkansas for Medical Sciences
(501) 603-1239


-----Original Message-----
From: Pavel Kaspler [mailto:pkaspler@uhnres.utoronto.ca]
Sent: Thursday, May 23, 2002 2:16 PM
To: MontagueDonnaC@exchange.uams.edu
Subject: GFP fluorescence after formalin/paraffin


Dear Dr. Montague,

Sorry to disturb you, but I have learnt you have experience RE issue I
am highly interested in.
I need to get GFP fluorescence survived in sections which underwent
routine pathology processing : formalin fixation, paraffin embedding and

H&E staining.

I you have any information about that, like your own experience, another

persons, references, I would appreciate it very much.
Also, is GFP kind essential? Are they some tricks with filters used to
cut off non-specific background?

Thank you for advance,
Pavel





<< Previous Message | Next Message >>