I have been helped by Histonet concerning the aldehyde-fuchsin for stain
pancreatic beta cell granules. Thanks. Now, I am able to get stained beta
cell granules using Gomori method. If the tissues fixed by 10% formalin,
I am able to stain Beta cell granules. However, my tissues were fixed by
Zinc fixative. The Zinc fixative is purchased from Pharmingen. I am not
able to stain these pancreatic Beta cell granules. Any clue?
Zinc Fixative buffer (1000 ml):
0.1 M Tris Buffer ph=7.4
0.5 g Calcium Acetate
5.0 g Zinc Acetate
5.0 g Zinc Chloride
Cardion Pharmaceutical Inc.
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