silicone membrane sections
I have had a request to cut sections from a novel porous silicone
discs onto which is grown ciliated epithelium.
The silicone membrane is made buy mixing a silicone plastic with fine
salt grains and extruding the mixture through a circular dye to form
These cylinders are cut into circular discs.
The discs are washed in water to remove the salt which leaves an open
mesh network of the plastic.
Fibroblasts are grown on the discs and then ciliated epithelium is
grown on the fibroblasts.
The object is to cut sections to see the penetration of cells into
the open structure of the disc.
I have tried processing into wax but the plastic is too soft and it
pulls away from the wax.
Soaking the disc in gelatine and fixing before processing does not
help either because the plastic remains flexible.
I have tried frozen sections but again the plastic remains flexible
at -20C and will not section.When looking at the sections down the
microscope is is clear that the plastic is pulling away from the wax.
Freezing in liquid nitrogen makes the discs stiff but we don't have a
cryostat which will get down to those temperatures.
Does anyone know of a way to harden the plastic without artifactually
damaging the cells grown upon it?
I would love to here from workers who have solved this problem.
Sheffield Children's Hospital UK
Do You Yahoo!?
Everything you'll ever need on one web page
from News and Sport to Email and Music Charts
<< Previous Message | Next Message >>