re: freezing antibodies
My method of freezing and storing Abs:
First I look to see if the antibody data sheet from the manufacturer states
that the antibody HAS to be stored frozen. If that is the case, then I work
up the antibody (while it's in the unfrozen state at arrival), to determine
the optimal antibody dilution, et al. Once I know the optimal dilution, I
know what volume of an antibody aliquot I'd mostly likely need to mix up a
fresh working dilution in the future.
For instance, if I work up an antibody and find that a dilution of 1:50
works best, then I know that whenever I mix up 1 ml of working dilution I
will need 20 ul of antibody.... so..... I then put aliquots of 25 ul each
into microcentrifuge tubes and store them in a Sarstedt cardboard box in the
freezer. I always pipet 5 more uls into each microcentrifuge tube than I
will need in the future, because when I go to retrieve (pipet) the antibody
out of the tube at a later date, I can never pipet out every single ul.
Thus, the reason for pipeting in a little more than I need.
The few ul remainder is put back in the freezer box with a red dot on the
top of the vial, telling me that it has been thawed once already. These are
held as emergency back-ups, should I ever run out of Ab before my next
shipment comes in.
This may not be terribly cost-efficient, but it is easier for me to waste a
few uls here and there than to add buffer to the vial and attempt to flush
out all the antibody that has clung to the side of the vial.
U of MN Vet Diag Lab
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