bovine tendon processing
Does anyone have tricks for processing and
sectioning bovine tendon that they would like to share? I am
currently processing cross sections of tendon at 20 minutes each in 7 stations
of graded alcohols, 20 min. each in 2 xylene stations, and 35 min. each in 4
paraffin stations. The tissue is fixed overnight in 10%
Before sectioning the blocks are
soaked in Mollifex between 20-60 minutes. The tissue
remains as hard as a rock, and the sections are shattered. A day after
processing the specimen has a slight caved in appearance of under dehydration,
but because the tissue is already so hard I am having a hard time believing that
I need to increase my alcohol times.
Any help would be greatly appreciated.
Thanks so much.
Integra LifeSciences, CRC
San Diego CA
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