Re: all you huffers
I believe and it is my experience that the paraffin
and the state of the tissue (dehydrated) protects the
paraffin section from RNase activity. I would worry
more about slides, waterbaths, hands, dust, and for
the most part reagents and clean technique. Sections
are at greatest risk of contamination after
deparaffinization & rehydration. I prefer to store my
paraffin unstained sections for ISH (DNA & RNA) in the
ribbon section with only air drying or at most 37C. I
have experienced loss of reactivity of mRNA in
sections stored for several months after initial
58-62C drying. This has also been observed with some
antigens for IHC, and some special histochemical
stains. However, the worse threat to unstained
sections, as I once heard in a story from a person who
worked in Texas- was that the sections had been eaten
by cockroaches while being stored in a lab drawer. My
advice is: Feel free to huff & puff, keep the
sections in the paraffin until staining, practice good
clean lab technique, and watch out for the
cockroaches.
Tim Plummer
PS: I am a huffer & puffer and have passed it on to
several others. I learned the method during my student
internship from a Med. Tech who was a supervisor at
the Minneapolis VA.
--- ajennings@unmc.edu wrote:
>
> Do any of you do RNA in situs? just wondering the
> affect of huffing on a
> block :-)
>
>
__________________________________________________
Do You Yahoo!?
Yahoo! Health - your guide to health and wellness
http://health.yahoo.com
<< Previous Message | Next Message >>