Re: Wenger's Method (pancreas)
"Gao, Yan" wrote:
> Does anyone have experience in stain
> Pancreatic B cell Granules. I used
> Wenger's method ...
> ... Any suggestions? Thanks
With traditional histochemistry it amounts to
histochemical detection of a high concentration
of -S=S- (cystine) in insulin. An oxidizing
agent such as acidic permanganate is followed
by a cationic dye at pH < 1. The dye sticks
firmly to the oxidation product (a cysteic acid).
There's plenty of peer-reviewed literature.
If you need some, ask.
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London, Canada N6A 5C1
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