Re: Wenger's Method (pancreas)

From:"J. A. Kiernan"

"Gao, Yan" wrote:
> Does anyone have experience in stain 
> Pancreatic B cell Granules.  I used
> Wenger's method ...
> ... Any suggestions?  Thanks

With traditional histochemistry it amounts to 
histochemical detection of a high concentration
of -S=S- (cystine) in insulin. An oxidizing 
agent such as acidic permanganate is followed 
by a cationic dye at pH < 1. The dye sticks 
firmly to the oxidation product (a cysteic acid). 
There's plenty of peer-reviewed literature. 
If you need some, ask.

John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1

<< Previous Message | Next Message >>