Re: OCT4 question
For IHC I prefer Immunocal (from Decal Chemical), this is formic acid
(5%). It works much quicker than EDTA and antigens are not adversely
affected in my hands.
"Dr. Reinhard von Wasielewski" wrote:
> High histonetters,
> two questions:
> First: I am looking for an antibody against OCT4/OCT3 that works in
> paraffin. Any suggestions ?
> Second: What is your favourite method for decalification if
> immunostains are to be made (bone metastasis of a breast carcinoma)?
> Thanks in advance.
> Reinhard von Wasielewski, MD
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