Re: Calcium Salts stain of decalcified bone

From:"J. A. Kiernan"

"CRME (Criss Meligro)" wrote:
> ... we are looking for the best way to show calcium salts in formalin fixed
> paraffin embedded decalcified bone....have tried the Von Kossa's method but
> we are looking for another choice or suggestions on the best method of
> decalcification to demonstrate these salts.   Thanks!

If you decalcify the specimen you will remove the
calcium salts and will be unable to stain them
with any technique.
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1

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