"CRME (Criss Meligro)" wrote:
> ... we are looking for the best way to show calcium salts in formalin fixed
> paraffin embedded decalcified bone....have tried the Von Kossa's method but
> we are looking for another choice or suggestions on the best method of
> decalcification to demonstrate these salts.   Thanks!

If you decalcify the specimen you will remove the
calcium salts and will be unable to stain them
with any technique.
-- 
-------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan@uwo.ca
   http://publish.uwo.ca/~jkiernan/