Edith,

I have not tried this protocol, but the results in the
publication suggest that it may work.  Good luck if
you try.

Control of Autofluorescence of Archival
Formaldehyde-fixed, Paraffin-embedded Tissue in
Confocal Laser Scanning Microscopy (CLSM)
Baschong etal
J Histochemistry & Cytochemistry
49(12):1565-1571, 2001
WWW.JHC.org

Tim Plummer
Transplantation Biology Research
Mayo Clinic
Rochester, MN USA

--- "Hines, Edith M."  wrote:
> >         I am having some difficulty overcoming
> background staining on
> > formalin-fixed, paraffin-embedded, human lung
> tissue sections.  I have
> tried blocking
> > the tissues with chromotrope 2R, and with ammonium
> chloride.  Both of
> these
> > methods have not significantly reduced the amount
> of autofluorescence that
> I
> > observe.
> >          Any advice that may be able to provide
> would be greatly
> > appreciated.
> > 
> > Thank you for your time,
> > Edie Hines
> > Research Technologist
> > Mayo Clinic Scottsdale
> > hines.edith@mayo.edu
> > 
> 
> 
> 


__________________________________________________
Do You Yahoo!?
LAUNCH - Your Yahoo! Music Experience
http://launch.yahoo.com