Re: Anti-GFP staining in paraffin sections
Re: Anti-GFP staining in paraffin
sections
I've used Anti-Green Fluorescent
Protein Rabbit IgG Fraction from Molecular Probes #A-11122 as a
primary Ab, then Rabbit IgG whole molecule unconjugated from Jackson
ImmunoResearch 011-000-003 as secondary Ab and the Rabbit/Peroxidase
EnVision+ System Kit from DAKO #K-4010 or K4011 (difference is size)
for detection. Works very well.
The protein (the P part of GFP)
was originally from the jellyfish Aequorea victoria . If you do a web search for that name you will
get some very cool pictures.
Susan Bell
Research Assistant
Targeted Genetics Corporation
1100 Olive Way Suite 100
Seattle WA 98101
Phone: 206-521-4830
Fax: 206-223-0288
bells@targen.com
At 2:30 PM -0400 5/15/02, Sarka Lhotak wrote:
Hello Netters,
Has anybody done a peroxidase staining against
GFP in paraffin
tissue sections? I am wondering whether the antibody can bind even to
GFP
that is no longer fluorescent (due to alcohol processing etc.)?
Thanks a lot,
Sarka Lhotak
Hamilton Regional Cancer
Centre
McMaster University
Hamilton, Ontario, Canada
--
Susan Bell
Research Assistant
Targeted Genetics Corporation
1100 Olive Way Suite 100
Seattle WA 98101
Phone: 206-521-4830
Fax: 206-223-0288
bells@targen.com
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