Re: Anti-GFP staining in paraffin sections

From:nina leek


It's Green Fluorescent Protein, originally found in jellyfish, and now,
through genetic engineering, available in other colors as well.  Also through
genetic engineering, it can be introduced into the genomes of other species,
accompanied by genes for other proteins of interest.  This means you can
screen the organisms you are playing with for the "other protein of interest"
by looking for the fluorescence of the GFP, which is now (genetically) tied
to it.  A very neat trick.

I'm not sure why someone would want to stain for it in (presumably) FFPE
sections, but they may be expansive enough to tell us.

Adrian Leek.

Cathy Gorrie wrote:

> What's GFP?
> At 2:30 PM -0400 15/5/02, Sarka Lhotak wrote:
> >Hello Netters,
> >       Has anybody done a peroxidase staining against GFP in paraffin
> >tissue sections? I am wondering whether the antibody can bind even to GFP
> >that is no longer fluorescent (due to alcohol processing etc.)?
> >       Thanks a lot,
> >
> >Sarka Lhotak
> >
> >Hamilton Regional Cancer Centre
> >McMaster University
> >Hamilton, Ontario, Canada

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