RE: embryonic whole mounts

From:Ralph Marcucio


The Gold Standard staging series for avian embryos is Hamburger and Hamilton
(1951) Journal of Morphology 88:49-92.  We typically remove embryos from
eggs, and fix in 4% paraformaldehyde, then dehydrate to 100%EtOH and
photograph.  I'm not sure if this is the info. you were after.  Let me know
if you need more specific information or further help.

Ralph Marcucio

Ralph Marcucio, PhD
Department of Orthopaedic Surgery
University of California at San Francisco
San Francisco, Ca 94143

Phone: 415-502-4945
Fax: 415-476-1128

-----Original Message-----
From: Marshall, Sharon, []
Sent: Thursday, May 09, 2002 12:54 AM
Subject: embryonic whole mounts

Hi histonetters,
I would like to make embryonic whole mounts of chick to show
developmental stages. Does anybody have a reliable method or
sharon marshall
Dept. Human Biology
University of Cape Town
Cape Town
South Africa
Sharon Marshall (Medical Technologist)
Post-Graduate Histology Lab.
Dept. of Human Biology
University of Cape Town
South Africa

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