Nancy

I recently purchased an anti-caspase 3 active form from BioVision, Cat
number 3015-100. Works extremely well on FFPE material. I use high grade
glioma as a control, EDTA antigen retrieval and overnight incubation at
1/500.

Cheers

Jim Manavis
Senior Hospital Scientist
Department of Neuropathology
Institute of Medical & Veterinary Science
Adelaide, SA, 5000
Australia
Phone: 61-08-8222-3668
FAX: 61-08-8222 3204
e.mail: jim.manavis@imvs.sa.gov.au
Disclaimer: Not this little black duck!


-----Original Message-----
From: Nperson211@aol.com [mailto:Nperson211@aol.com]
Sent: Tuesday, 14 May 2002 12:54 AM
To: histonet@pathology.swmed.edu
Subject: apoptosis


Histonetters,
Does anyone have a strong recommendation for an antibody to ssDNA
that works well in FFPE?  I am interested in having two reliable
methods that demonstrate apoptosis.  I have Apotag kits but would
like some other possiblities as well.  If you have protocols I
would appreciate those, too.
Thanks very much,
Nancy Lemke
Hermelin Brain Tumor Center
Henry Ford Hospital
Detroit, MI