RE: Ki-67

From:Jeff Silverman

Joost- Refrigeration can mitigate the antigenicity loss, higher storage
temperatures hasten the loss. Here's a eeference with the author's email-
pperhaps he (she) can send you a reprint. Use fresh sections. Appl
Immunohistochem Mol Morphol 2000 Mar;8(1):61-70 Paraffin section storage and
immunohistochemistry. Effects of time, temperature, fixation, and retrieval
protocol with emphasis on p53 protein and MIB1 antigen. Wester K, Wahlund E,
Sundstrom C, Ranefall P, Bengtsson E, Russell PJ, Ow KT, Malmstrom PU, Busch
C.  Department of Genetics, Uppsala University, Sweden.

Jeff Silverman HT HTL QIHC (ASCP)
Southside Hospital
Bay Shore NY

-----Original Message-----
From: Bruyntjes, J.P. []
Sent: Wednesday, May 01, 2002 2:52 AM
To: 'Jeff Silverman'
Subject: Ki-67

Jim- Make sure you use freshly cut sections for Ki67. If you use sections
that have been stored precut for long periods, Ki67 is one of those nuclear
antigens who immunoreactivity degrades on storage.
Jeff Silverman HT HTL QIHC (ASCP)
Southside Hospital
Bay Shore NY USA

Hi Jeff

I would like to ask you a question about Ki-67. You just told someone on that, when you want to stain for this antibody
you have to use freshly cut slides. I have had about the samne trouble, but
somehow I managed to stain the 'old' slides as well using the high pressure
cooker. But can you tell me when do these problems occur, after how long
will these problems occur?

Joost Bruijntjes
TNO Nutrition and Food Research
Phone + 31 30 6944483

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