RE: Cytokine IHC

From:"Monson, Frederick C."

Morning Tom,
	Here's a good place to start looking.

I searched Google for:  .

You are about to seek an answer to one of the great immunologic questions.
"Is a monoclonal antibody REALLY epitope specific?"  If it is, then it will
work - selectively and specifically - everywhere.


Fred Monson

Frederick C. Monson, PhD   
Center for Advanced Scientific Imaging
Schmucker II Science Center
West Chester University
South Church Street and Rosedale
West Chester, Pennsylvania, USA, 19383
Phone:  610-738-0437
FAX:  610-738-0437
Visitors URL:

> ----------
> From:
> Sent: 	Tuesday, May 14, 2002 4:35 PM
> To: 	HistoNet Server
> Subject: 	Cytokine IHC
> Hello Histonetters, 
> I am seeking advice for detecting TNF alpha, IFN gamma, and IL-2 in frozen
> mouse tissues (OCT blocks) using IHC methods.  Is this possible, and if
> so, what are the optimal conditions for detection?  Also, antibody sources
> would be very helpful. 
> Thank you all for your help. 
> Tom Crowell 
> Biogen, Inc. 
> Cambridge, MA 

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