Bryan,	

You are still allowed mercury in your lab? That is really surprising since
there is absolute zero tolerance for mercury in this part of the Great
Lakes. I'm mean blood pressure cuffs, thermometers, everything. I was under
the impression that Canadian environmental law was pretty strict. Our docs
certainly preferred B-5 but the Western Lake Superior Sanitary District put
the hammer down!  We use a very good substitute(B-plus)and I'm glad we do
for the sake of the environment.

> -----Original Message-----
> From:	Bryan Hewlett [SMTP:bhewlett@cogeco.ca]
> Sent:	Thursday, May 09, 2002 5:56 AM
> To:	Sharon Cassar; HistoNet Server
> Subject:	Re: B5
> 
> Sharon,
> 
> B-5 Fixative.
> Sodium acetate mercuric chloride formalin, pH 5.8-6.0.
> 
> Distilled water......90 mL
> Mercuric chloride...6 g
> Sodium acetate(anhyd.)..1.25g
> 
> Mix well, label as B-5 stock.
> Immediately prior to use add 10 mL 37-40% formaldehyde and mix.
> 
> Fix 2-3 mm slice of L.N. for 4-6 hours(K=0.8)
> Rinse in 3 changes NBF, 5 min. each and transfer to NBF. Process.
> Lillie suggests 12-24 hours, and adds that 5-7 days does not overharden!
> 
> Reference:
> Lillie,R.D. Histopathologic Technic and Practical Histochemistry, 3rd Ed.
> 1965
> 
> Bryan
> 
> Bryan R. Hewlett
> Technical Specialist
> Anatomical Pathology
> Hamilton Regional Laboratory Medicine Program
> Ontario, Canada
> 
> 
> 
> ----- Original Message -----
> From: "Sharon Cassar" 
> To: "HistoNet Server" 
> Sent: Wednesday, May 08, 2002 11:08 PM
> Subject: B5
> 
> 
> > Hello
> >
> > Can anyone send me the formulation and the procedure of B5 fixative on
> fresh
> > lymph nodes.
> >
> > Thank you
> >
> > Sharon Cassar
> > Senior MLS
> >
> >
> >
> 
>