RE: Autofluorescence on Human Lung Tissue
Thank you to all of you who offered advice.
I am going to try several of the suggested methods and I will inform you how
Mayo Clinic Scottsdale
From: Tim Plummer [mailto:firstname.lastname@example.org]
Sent: Tuesday, May 14, 2002 1:45 PM
To: Hines, Edith M.; 'HistoNet Server'
Subject: Re: Autofluorescence on Human Lung Tissue
I have not tried this protocol, but the results in the
publication suggest that it may work. Good luck if
Control of Autofluorescence of Archival
Formaldehyde-fixed, Paraffin-embedded Tissue in
Confocal Laser Scanning Microscopy (CLSM)
J Histochemistry & Cytochemistry
Transplantation Biology Research
Rochester, MN USA
--- "Hines, Edith M." wrote:
> > I am having some difficulty overcoming
> background staining on
> > formalin-fixed, paraffin-embedded, human lung
> tissue sections. I have
> tried blocking
> > the tissues with chromotrope 2R, and with ammonium
> chloride. Both of
> > methods have not significantly reduced the amount
> of autofluorescence that
> > observe.
> > Any advice that may be able to provide
> would be greatly
> > appreciated.
> > Thank you for your time,
> > Edie Hines
> > Research Technologist
> > Mayo Clinic Scottsdale
> > email@example.com
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