RE: Anti-GFP staining in paraffin sections
I recently started using the anti-GFP form NeoMarkers (clone gfp01). Works
well in FFPE tissues. Do not know if the GFP was still fluorescent (Didn't
check)
-----Original Message-----
From: Sarka Lhotak [mailto:lhotaks@mcmail.cis.mcmaster.ca]
Sent: Wednesday, May 15, 2002 2:31 PM
To: histonet@pathology.swmed.edu
Subject: Anti-GFP staining in paraffin sections
Hello Netters,
Has anybody done a peroxidase staining against GFP in paraffin
tissue sections? I am wondering whether the antibody can bind even to GFP
that is no longer fluorescent (due to alcohol processing etc.)?
Thanks a lot,
Sarka Lhotak
Hamilton Regional Cancer Centre
McMaster University
Hamilton, Ontario, Canada
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