RE: Alkaline phosphatase

From:Dan Beniker

Also, check out: "Histochemical Localization of AP activity in decalcified
bone and Cartilage" by Dengshun Miao and Andrew Scutt in the Journal of
Histochemistry and Cytochemistry Volume 50(3): 333-340, 2002

They use a funky fixative and do a series of glycerol/sucrose infiltration
steps in conjunction with their EDTA decal, but they report good results!

> -----Original Message-----
> From:	Karen M Majahad []
> Sent:	Friday, May 03, 2002 1:45 PM
> To:
> Subject:	Alkaline phosphatase
> Has anyone had any luck with staining for alkaline
> phosphotase in paraffin sections. I was told that it is very
> hard to do enzyme staining on paraffin sections.

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