Decalcification methods for immunostainings

From:"Dr. Reinhard von Wasielewski"

Hi Histonetters
we perform a lot of immunostainings on decalcified bone marrows 
(Jamshidi etc). Steroid-receptors and HER2 is hampered by harsh 
decalcification as far as we know.
What do you think are the most common methods for decalcification of 
bone metastases so that you still can perform reliable ER, PR and 
HER2 testings ? A detailed name or vendor of decal -fluids is very 
much appreciated !
Thanks in advance !
Reinhard von Wasielewski
Immunolab of Hannover Medical School, Germany.
Reinhard von Wasielewski, MD

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