Hello all,

Does everyone know exactly how the DAPI (blue fluorecence) counter staining 
work?

I ran the IFC for one antibody that is pretreated with 2N HCl and another 
antibody without any pretreatment and for some reason the slides that is not 
pretreated have a VERY bright blue DAPI counterstainig (very nice). I 
brought it from Sigma (cat. # D9542).

Can anyone explain to me how this DAPI worked in the nucleus?

Many thanks if anyone can help.

Jasmine



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