Does everyone know exactly how the DAPI (blue fluorecence) counter staining
I ran the IFC for one antibody that is pretreated with 2N HCl and another
antibody without any pretreatment and for some reason the slides that is not
pretreated have a VERY bright blue DAPI counterstainig (very nice). I
brought it from Sigma (cat. # D9542).
Can anyone explain to me how this DAPI worked in the nucleus?
Many thanks if anyone can help.
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