Cytokine IHC

Hello Histonetters,

I am seeking advice for detecting TNF alpha, IFN gamma, and IL-2 in frozen mouse tissues (OCT blocks) using IHC methods.  Is this possible, and if so, what are the optimal conditions for detection?  Also, antibody sources would be very helpful.

Thank you all for your help.

Tom Crowell
Biogen, Inc.
Cambridge, MA
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