Autofluorescence on Human Lung Tissue
> I am having some difficulty overcoming background staining on
> formalin-fixed, paraffin-embedded, human lung tissue sections. I have
tried blocking
> the tissues with chromotrope 2R, and with ammonium chloride. Both of
these
> methods have not significantly reduced the amount of autofluorescence that
I
> observe.
> Any advice that may be able to provide would be greatly
> appreciated.
>
> Thank you for your time,
> Edie Hines
> Research Technologist
> Mayo Clinic Scottsdale
> hines.edith@mayo.edu
>
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