mouse perfusion


Has anyone out there in histoland ever heard of perfusing mice with Zinc 
Formalin instead of 4% paraformaldehyde?  Where I work at, they perfuse mice in 
4% paraformaldehyde, then fix tissue overnight in 4%para in refrigerator and 
then give it to me to process; it gets processed with zinc formalin.  I would 
think 4%para. would not be stable in tissue processor because its kept at room 
temperature overnight.  4% Paraformaldehyde is so expensive and the stability 
is less than zinc formalin(which is kept at room temp).  Going from 
refrigerator temp to room temp constantly can not be good. Any comments, 
suggestion, or advice would be greatly appreciated. 


Dina D. Gaupp

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