mouse perfusion
Hi,
Has anyone out there in histoland ever heard of perfusing mice with Zinc
Formalin instead of 4% paraformaldehyde? Where I work at, they perfuse mice in
4% paraformaldehyde, then fix tissue overnight in 4%para in refrigerator and
then give it to me to process; it gets processed with zinc formalin. I would
think 4%para. would not be stable in tissue processor because its kept at room
temperature overnight. 4% Paraformaldehyde is so expensive and the stability
is less than zinc formalin(which is kept at room temp). Going from
refrigerator temp to room temp constantly can not be good. Any comments,
suggestion, or advice would be greatly appreciated.
Thanks,
Dina D. Gaupp
dgaupp@tulane.edu
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