endogenous peroxidase

From:"t.hacker@har.mrc.ac.uk" <T.Hacker@har.mrc.ac.uk>

What are you using for blocking endogenous peroxidase in acetone 
fixed frozen sections?
I have used a weak solution of H202 which is not always effective, 
too strong and I get excessive "bubbling" of the tissue. Yes, I 
know, try a strength in between, but I was wondering if there is 
something more appropriate and less aggressive.
I look forward to a wealth of knowledge and experience, preferably 
by 9am GMT tomorrow.
Many thanks,
Terry.
Terry Hacker,
Medical Research Council,
Harwell,
Didcot,
Oxfordshire, OX11 ORD
01235 834393 x360



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